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Patch Clamping Technique Simplified |
Article Submitted by: Marcia Henin
Sunday, 24 January 2010
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Patch Clamping is a process, which determines what's happening in a single living cell. The technique used for this measurement is a patch clamp that requires a fine pipette held tightly opposite the cell membrane. A fine pipette is derived by heating and pulling a quartz capillary tube or small glass. When the heated glass is pulled by a machine, the tip derived is smaller than a human hair, and that is equivalent to one micron. The pipette derived is hollow and it can be used to blow or suck which depends on the experiment performed. The patch clamping process is done by machine or some scientists also do it by mouth. The pipette and cell membrane should make a good contact to measure cell activity accurately. A single frog mytocyte is picked by sucking on the pipette. The good contact enables to record ion channels opening and closing. What is Patch Clamp? Patch clamp contains an electrode inside a glass pipette. The pipette consists of a salt solution that resembles the fluid generally found within the cell. The pipette is lowered to the cell membrane where it forms a tight seal with the cell. As soon as little suction is applied to the pipette, the "patch" of the membrane within the pipette bursts allowing access to the entire cell. . The electrode that is connected to a specialized circuitry can be used to measure currents passing through cells' ion channels. Ion channels are actually transmembrane pores, which allow the ions to pass in and out of the cell through the elctrochemical gradient. There are several ion channels that are distinguished upon their gating mechanism, ion selectivity and sequence similarity. The electrical circuitry and the "clamp" can be used to check membrane potential to any voltage. Such an arrangement comes in handy when we need to measure activity of voltage dependent channels. Article Source: http://www.ArticleBlast.com |
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